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These cells exhibit a variety of markers characteristic of extravillous invasive trophoblast cells in situ. CRL-3271 HTR-8SVneo cells were derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
Insulin-like growth factor IGF-II NDOG-5 proliferating cell nuclear antigen PCNA.
. For cultures that require storage in liquid nitrogen it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying.
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